Find here an updated list of our publications
Per-pixel unmixing of spectrally overlapping fluorophores using intra-exposure excitation modulation
TALANTA
2024
DOI: 10.1016/j.talanta.2023.125397
Valenta, Hana; Bierbuesse, Franziska; Vitale, Raffaele; Ruckebusch, Cyril; Vandenberg, Wim; Dedecker, Peter
Evidence of Cytolysin A nanopore incorporation in mammalian cells assessed by a graphical user interface
NANOSCALE
2023
DOI: 10.1039/d3nr01977b
Lucas, Florian Leonardus Rudolfus; Finol-Urdaneta, Rocio K.; Van Thillo, Toon; Mcarthur, Jeffrey R.; van der Heide, Nieck Jordy; Maglia, Giovanni; Dedecker, Peter; Strauss, Olaf; Wloka, Carsten
Quantitative determination of the full switching cycle of photochromic fluorescent proteins
CHEMICAL COMMUNICATIONS
2023
DOI: 10.1039/d3cc01617j
Bourges, Anais C.; Moeyaert, Benjamien; Bui, Thi Yen Hang; Bierbuesse, Franziska; Vandenberg, Wim; Dedecker, Peter
Live-Cell SOFI Correlation with SMLM and AFM Imaging
ACS BIO \& MED CHEM AU
2023
DOI: 10.1021/acsbiomedchemau.2c00086
Hargreaves, RileyB.; Duwe, Sam; Rozario, Ashley M.; Funston, Alison M.; Tabor, Rico F.; Dedecker, Peter; Whelan, Donna R.; Bell, Toby D. M.
Oxygen-induced chromophore degradation in the photoswitchable red fluorescent protein rsCherry
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
2023
DOI: 10.1016/j.ijbiomac.2023.124179
Bui, Thi Yen Hang; De Zitter, Elke; Moeyaert, Benjamien; Pecqueur, Ludovic; Srinivasu, Bindu Y.; Economou, Anastassios; Fontecave, Marc; Van Meervelt, Luc; Dedecker, Peter; Pedre, Branden
An unusual disulfide-linked dimerization in the fluorescent protein rsCherryRev1.4
ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS
2023
DOI: 10.1107/S2053230X23000572
Bui, Thi Yen Hang; Dedecker, Peter; Van Meervelt, Luc
Modifying PCDH19 levels affects cortical interneuron migration
FRONTIERS IN NEUROSCIENCE
2022
DOI: 10.3389/fnins.2022.887478
Pancho, Anna; Mitsogiannis, Manuela D. D.; Aerts, Tania; Dalla Vecchia, Marco; Ebert, Lena K. K.; Geenen, Lieve; Noterdaeme, Lut; Vanlaer, Ria; Stulens, Anne; Hulpiau, Paco; Staes, Katrien; Van Roy, Frans; Dedecker, Peter; Schermer, Bernhard; Seuntjens, Eve
Synchronized, Spontaneous, and Oscillatory Detachment of Eukaryotic Cells: A New Tool for Cell Characterization and Identification
ADVANCED SCIENCE
2022
DOI: 10.1002/advs.202200459
Yongabi, Derick; Khorshid, Mehran; Losada-Perez, Patricia; Sichani, Soroush Bakhshi; Jooken, Stijn; Stilman, Wouter; Thesseling, Florian; Martens, Tobie; Van Thillo, Toon; Verstrepen, Kevin; Dedecker, Peter; Vanden Berghe, Pieter; Lettinga, Minne Paul; Bartic, Carmen; Lieberzeit, Peter; Schoning, Michael J.; Thoelen, Ronald; Fransen, Marc; Wubbenhorst, Michael; Wagner, Patrick
Absolute measurement of cellular activities using photochromic single-fluorophore biosensors and intermittent quantification
NATURE COMMUNICATIONS
2022
DOI: 10.1038/s41467-022-29508-w
Bierbuesse, Franziska; Bourges, Anais C.; Gielen, Vincent; Monkemoller, Viola; Vandenberg, Wim; Shen, Yi; Hofkens, Johan; Vanden Berghe, Pieter; Campbell, Robert E.; Moeyaert, Benjamien; Dedecker, Peter
Multilinear Slicing for curve resolution of fluorescence imaging with sequential illumination
TALANTA
2022
DOI: 10.1016/j.talanta.2022.123231
Cevoli, Dario; Hugelier, Siewert; Van den Eynde, Robin; Devos, Olivier; Dedecker, Peter; Ruckebusch, Cyril
Nanoscale characterization of drug-induced microtubule filament dysfunction using super-resolution microscopy
BMC BIOLOGY
2021
DOI: 10.1186/s12915-021-01164-4
Rozario, Ashley M.; Duwe, Sam; Elliott, Cade; Hargreaves, Riley B.; Moseley, Gregory W.; Dedecker, Peter; Whelan, Donna R.; Bell, Toby D. M.
Separation of spectrally overlapping fluorophores using intra-exposure excitation modulation
BIOPHYSICAL REPORTS
2021
DOI: 10.1016/j.bpr.2021.100026
Valenta, Hana; Hugelier, Siewert; Duwe, Sam; Lo Gerfo, Giulia; Mueller, Marcel; Dedecker, Peter; Vandenberg, Wim
Self-contained and modular structured illumination microscope
BIOMEDICAL OPTICS EXPRESS
2021
DOI: 10.1364/BOE.423492
Van den Eynde, Robin; Vandenberg, Wim; Hugelier, Siewert; Bouwens, Arno; Hofkens, Johan; Mueller, Marcel; Dedecker, Peter
Actuation enhances patterning in human neural tube organoids
NATURE COMMUNICATIONS
2021
DOI: 10.1038/s41467-021-22952-0
Fattah, Abdel Rahman Abdel; Daza, Brian; Rustandi, Gregorius; Berrocal-Rubio, Miguel Angel; Gorissen, Benjamin; Poovathingal, Suresh; Davie, Kristofer; Barrasa-Fano, Jorge; Condor, Mar; Cao, Xuanye; Rosenzweig, Derek Hadar; Lei, Yunping; Finnell, Richard; Verfaillie, Catherine; Sampaolesi, Maurilio; Dedecker, Peter; Van Oosterwyck, Hans; Aerts, Stein; Ranga, Adrian
Design of experiments for the optimization of SOFI super-resolution microscopy imaging
BIOMEDICAL OPTICS EXPRESS
2021
DOI: 10.1364/BOE.421168
Cevoli, Dario; Vitale, Raffaele; Vandenberg, Wim; Hugelier, Siewert; Van den Eynde, Robin; Dedecker, Peter; Ruckebusch, Cyril
Smoothness correction for better SOFI imaging
SCIENTIFIC REPORTS
2021
DOI: 10.1038/s41598-021-87164-4
Hugelier, Siewert; Vandenberg, Wim; Lukes, Tomas; Grussmayer, Kristin S.; Eilers, Paul H. C.; Dedecker, Peter; Ruckebusch, Cyril
Simultaneous readout of multiple FRET pairs using photochromism
NATURE COMMUNICATIONS
2021
DOI: 10.1038/s41467-021-22043-0
Roebroek, Thijs; Vandenberg, Wim; Sipieter, Francois; Hugelier, Siewert; Stove, Christophe; Zhang, Jin; Dedecker, Peter
Structure-Function Dataset Reveals Environment Effects within a Fluorescent Protein Model System
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
2021
DOI: 10.1002/anie.202015201
De Zitter, Elke; Hugelier, Siewert; Duwe, Sam; Vandenberg, Wim; Tebo, Alison G.; Van Meervelt, Luc; Dedecker, Peter
Photochromic Fluorophores Enable Imaging of Lowly Expressed Proteins in the Autofluorescent Fungus Candida albicans
MSPHERE
2021
DOI: 10.1128/mSphere.00146-21
Van Genechten, Wouter; Demuyser, Liesbeth; Duwe, Sam; Vandenberg, Wim; Van Dijck, Patrick; Dedecker, Peter
Quantitative Model for Reversibly Photoswitchable Sensors
ACS SENSORS
2021
DOI: 10.1021/acssensors.0c02414
Adelizzi, Beatrice; Gielen, Vincent; Le Saux, Thomas; Dedecker, Peter; Jullien, Ludovic
Model-free pixelation correction in SOFI imaging
OSA CONTINUUM
2021
DOI: 10.1364/OSAC.413107
Bierbuesse, Franziska; Gielen, Vincent; Vandenberg, Wim; Dedecker, Peter
Orthogonal fluorescent chemogenetic reporters for multicolor imaging
NATURE CHEMICAL BIOLOGY
2021
DOI: 10.1038/s41589-020-0611-0
Tebo, Alison G.; Moeyaert, Benjamien; Thauvin, Marion; Carlon-Andres, Irene; Boken, Dorothea; Volovitch, Michel; Padilla-Parra, Sergi; Dedecker, Peter; Vriz, Sophie; Gautier, Arnaud
Identification of psychedelic new psychoactive substances (NPS) showing biased agonism at the 5-HT2AR through simultaneous use of β-arrestin 2 and miniGαq bioassays
BIOCHEMICAL PHARMACOLOGY
2020
DOI: 10.1016/j.bcp.2020.114251
Pottie, Eline; Dedecker, Peter; Stove, Christophe P.
`Live and Large’: Super-Resolution Optical Fluctuation Imaging (SOFI) and Expansion Microscopy (ExM) of Microtubule Remodelling by Rabies Virus P Protein
AUSTRALIAN JOURNAL OF CHEMISTRY
2020
DOI: 10.1071/CH19571
Rozario, Ashley M.; Zwettler, Fabian; Duwe, Sam; Hargreaves, Riley B.; Brice, Aaron; Dedecker, Peter; Sauer, Markus; Moseley, Gregory W.; Whelan, Donna R.; Bell, Toby D. M.
Genetically encoded biosensors based on innovative scaffolds
INTERNATIONAL JOURNAL OF BIOCHEMISTRY \& CELL BIOLOGY
2020
DOI: 10.1016/j.biocel.2020.105761
Moeyaert, Benjamien; Dedecker, Peter
Mechanistic Investigations of Green mEos4b Reveal a Dynamic Long-Lived Dark State
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
2020
DOI: 10.1021/jacs.0c01880
De Zitter, Elke; Ridard, Jacqueline; Thedie, Daniel; Adam, Virgile; Levy, Bernard; Byrdin, Martin; Gotthard, Guillaume; Van Meervelt, Luc; Dedecker, Peter; Demachy, Isabelle; Bourgeois, Dominique
QCM-D Study of Time-Resolved Cell Adhesion and Detachment: Effect of Surface Free Energy on Eukaryotes and Prokaryotes
ACS APPLIED MATERIALS \& INTERFACES
2020
DOI: 10.1021/acsami.0c00353
Yongabi, Derick; Khorshid, Mehran; Gennaro, Alessia; Jooken, Stijn; Duwe, Sam; Deschaume, Olivier; Losada-Perez, Patricia; Dedecker, Peter; Bartic, Carmen; Wubbenhorst, Michael; Wagner, Patrick
Presenting a codon-optimized palette of fluorescent proteins for use in Candida albicans
SCIENTIFIC REPORTS
2020
DOI: 10.1038/s41598-020-63308-w
Van Genechten, Wouter; Demuyser, Liesbeth; Dedecker, Peter; Van Dijck, Patrick
A comprehensive dataset of image sequences covering 20 fluorescent protein labels and 12 imaging conditions for use in super-resolution imaging
DATA IN BRIEF
2020
DOI: 10.1016/j.dib.2020.105273
Moeyaert, Benjamien; Dedecker, Peter
SOFIevaluator: a strategy for the quantitative quality assessment of SOFI data
BIOMEDICAL OPTICS EXPRESS
2020
DOI: 10.1364/BOE.382278
Moeyaert, Benjamien; Vandenberg, Wim; Dedecker, Peter
High-speed multiplane structured illumination microscopy of living cells using an image-splitting prism
NANOPHOTONICS
2020
DOI: 10.1515/nanoph-2019-0346
Descloux, Adrien; Mueller, Marcel; Navikas, Vytautas; Markwirth, Andreas; Van den Eynde, Robin; Lukes, Tomas; Huebner, Wolfgang; Lasser, Theo; Radenovic, Aleksandra; Dedecker, Peter; Huser, Thomas R.
Quantitative comparison of camera technologies for cost-effective super-resolution optical fluctuation imaging (SOFI)
JOURNAL OF PHYSICS-PHOTONICS
2019
DOI: 10.1088/2515-7647/ab36ae
Van den Eynde, Robin; Sandmeyer, Alice; Vandenberg, Wim; Duwe, Sam; Huebner, Wolfgang; Huser, Thomas; Dedecker, Peter; Mueller, Marcel
An extended quantitative model for super-resolution optical fluctuation imaging (SOFI)
OPTICS EXPRESS
2019
DOI: 10.1364/OE.27.025749
Vandenberg, Wim; Leutenegger, Marcel; Duwe, Sam; Dedecker, Peter
Mechanistic investigation of mEos4b reveals a strategy to reduce track interruptions in sptPALM
NATURE METHODS
2019
DOI: 10.1038/s41592-019-0462-3
De Zitter, Elke; Thedie, Daniel; Monkemoller, Viola; Hugelier, Siewert; Beaudouin, Joel; Adam, Virgile; Byrdin, Martin; Van Meervelt, Luc; Dedecker, Peter; Bourgeois, Dominique
Sensitive and specific detection of E. coli using biomimetic receptors in combination with a modified heat-transfer method
BIOSENSORS \& BIOELECTRONICS
2019
DOI: 10.1016/j.bios.2019.04.026
Cornelis, Peter; Givanoudi, Stella; Yongabi, Derick; Iken, Heiko; Duwe, Sam; Deschaume, Olivier; Robbens, Johan; Dedecker, Peter; Bartic, Carmen; Wubbenhorst, Michael; Schoening, Michael J.; Heyndrickx, Marc; Wagner, Patrick
The Persistence-Inducing Toxin HokB Forms Dynamic Pores That Cause ATP Leakage
MBIO
2018
DOI: 10.1128/mBio.00744-18
Wilmaerts, Dorien; Bayoumi, Mariam; Dewachter, Liselot; Knapen, Wouter; Mika, Jacek T.; Hofkens, Johan; Dedecker, Peter; Maglia, Giovanni; Verstraeten, Natalie; Michiels, Jan
Correcting for photodestruction in super-resolution optical fluctuation imaging
SCIENTIFIC REPORTS
2017
DOI: 10.1038/s41598-017-09666-4
Peeters, Yves; Vandenberg, Wim; Duwe, Sam; Bouwens, Arno; Lukes, Tomas; Ruckebusch, Cyril; Lasser, Theo; Dedecker, Peter
Reduced Fluorescent Protein Switching Fatigue by Binding-Induced Emissive State Stabilization
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
2017
DOI: 10.3390/ijms18092015
Roebroek, Thijs; Duwe, Sam; Vandenberg, Wim; Dedecker, Peter
Efficient switching of mCherry fluorescence using chemical caging
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
2017
DOI: 10.1073/pnas.1617280114
Cloin, Bas M. C.; De Zitter, Elke; Salas, Desiree; Gielen, Vincent; Folkers, Gert E.; Mikhaylova, Marina; Bergeler, Maike; Krajnik, Bartosz; Harvey, Jeremy; Hoogenraad, Casper C.; Van Meervelt, Luc; Dedecker, Peter; Kapitein, Lukas C.
Genetically encoded biosensors for visualizing live-cell biochemical activity at super-resolution
NATURE METHODS
2017
DOI: 10.1038/nmeth.4221
Mo, Gary C. H.; Ross, Brian; Hertel, Fabian; Manna, Premashis; Yang, Xinxing; Greenwald, Eric; Booth, Chris; Plummer, Ashlee M.; Tenner, Brian; Chen, Zan; Wang, Yuxiao; Kennedy, Eileen J.; Cole, Philip A.; Fleming, Karen G.; Palmer, Amy; Jimenez, Ralph; Xiao, Jie; Dedecker, Peter; Zhang, Jin
Effect of probe diffusion on the SOFI imaging accuracy
SCIENTIFIC REPORTS
2017
DOI: 10.1038/srep44665
Vandenberg, Wim; Dedecker, Peter
Complementarity of PALM and SOFI for super-resolution live-cell imaging of focal adhesions
NATURE COMMUNICATIONS
2016
DOI: 10.1038/ncomms13693
Deschout, Hendrik; Lukes, Tomas; Sharipov, Azat; Szlag, Daniel; Feletti, Lely; Vandenberg, Wim; Dedecker, Peter; Hofkens, Johan; Leutenegger, Marcel; Lasser, Theo; Radenovic, Aleksandra
Field-Controlled Charge Separation in a Conductive Matrix at the Single-Molecule Level: Toward Controlling Single-Molecule Fluorescence Intermittency
ACS OMEGA
2016
DOI: 10.1021/acsomega.6b00207
Kennes, Koen; Dedecker, Peter; Hutchison, James A.; Fron, Eduard; Uji-i, Hiroshi; Hofkens, Johan; Van der Auweraer, Mark
SOFI Simulation Tool: A Software Package for Simulating and Testing Super-Resolution Optical Fluctuation Imaging
PLOS ONE
2016
DOI: 10.1371/journal.pone.0161602
Girsault, Arik; Lukes, Tomas; Sharipov, Azat; Geissbuehler, Stefan; Leutenegger, Marcel; Vandenberg, Wim; Dedecker, Peter; Hofkens, Johan; Lasser, Theo
Sparse deconvolution of high-density super-resolution images
SCIENTIFIC REPORTS
2016
DOI: 10.1038/srep21413
Hugelier, Siewert; de Rooi, Johan J.; Bernex, Romain; Duwe, Sam; Devos, Olivier; Sliwa, Michel; Dedecker, Peter; Eilers, Paul H. C.; Ruckebusch, Cyril
Model-free uncertainty estimation in stochastical optical fluctuation imaging (SOFI) leads to a doubled temporal resolution
BIOMEDICAL OPTICS EXPRESS
2016
DOI: 10.1364/BOE.7.000467
Vandenberg, Wim; Duwe, Sam; Leutenegger, Marcel; Moeyaert, Benjamien; Krajnik, Bartosz; Lasser, Theo; Dedecker, Peter
RefSOFI for Mapping Nanoscale Organization of Protein-Protein Interactions in Living Cells
CELL REPORTS
2016
DOI: 10.1016/j.celrep.2015.12.036
Hertel, Fabian; Mo, Gary C. H.; Duwe, Sam; Dedecker, Peter; Zhang, Jin
Expression-Enhanced Fluorescent Proteins Based on Enhanced Green Fluorescent Protein for Super-resolution Microscopy
ACS NANO
2015
DOI: 10.1021/acsnano.5b04129
Duwe, Sam; De Zitter, Elke; Gielen, Vincent; Moeyaert, Benjamien; Vandenberg, Wim; Grotjohann, Tim; Clays, Koen; Jakobs, Stefan; Van Meervelt, Luc; Dedecker, Peter
Molecular Dynamic Indicators of the Photoswitching Properties of Green Fluorescent Proteins
JOURNAL OF PHYSICAL CHEMISTRY B
2015
DOI: 10.1021/acs.jpcb.5b04826
Smyrnova, Daryna; Moeyaert, Benjamien; Michielssens, Servaas; Hofkens, Johan; Dedecker, Peter; Ceulemans, Arnout